| Caspases play a key role in initiation of cellular events during apoptosis. The Caspase-7 Immunoassay Kit provides an effective immunosorbent enzyme assay for the specific, quantitative detection of caspase-7 activity in microtiter plates. The assay utilizes caspase-7 polyclonal antibody to capture activated caspase-7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated caspase-7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex 400nm and Em 505nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-7. Other known caspases and non-specific proteases are not detected. |
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| Catalog # | C2087-29B |
| Kit Components | • Cell Lysis Buffer |
| • Coating Buffer |
| • Anti-Caspase-7 Antibody (20X) |
| • Blocking Buffer |
| • Incubation Buffer |
| • DTT (1M) |
| • DEVD-AFC Substrate (1mM) |
| • Positive Control (rh-Caspase-7) |
| • Microtiter Plate |
| • Adhesive Plate Cover |
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| Related Products | |
| 1. Mitochondrial Apoptosis Detection Kit |
| 2. Mitochondria/Cytosol Fractionation and Cytochrome c Apoptosis Assay Kits |
| 3. ATP Determination Kit & ADP/ATP Ratio Assay Kit |
| 4. Glutathione and Nitric Oxide Detection Kits |
| 5. Annexin V-FITC, -Cy3, -Cy5, -EGFP, -PE, -Biotin Kits and Bulk Reagents |
| 6. Flow Cytometric Caspase Assay Kit |
| 7. Caspase-3 & -7 Immunoassay Kits |
| 8. Caspase-1,-2,-3,-5,-6,-8,-9,-10 Activity Assay Kits |
| 9. Active Human Caspase-1,-2,-3,-4,-5,-6,-7,-8,-9,-10 & Procaspase-3 |
| 10. Ready-to-use Caspase Inhibitors and Sets |
| 11. Bulk Caspase Substrates and Assay Buffers |
| 12. Calpain, Cathepsins, & Granzyme B Related Products |
| 13. Anti-PARP Monoclonal Antibody |
| 14. Anti-Caspase-1,-2,-3,-4,-5,-6,-7,-8,-9,-10,-11,-12,-13,-14 & Active Casp-3,-9 |
| 15. Anti-Apaf-1, Bcl-2, Bad, Bax, Bid, Bcl-x, Bim/Bod, Mcl-1, Cytochrome c |
| 16. Anti-DFF40, DFF45, FADD, DcR2, IRAK, ASK1, Wee1, CD40, p53, FLIP |
| 17. Other Apoptosis Related Antibodies |
| 18. Apoptotic DNA Ladder Detection Kit |
| 19. In Situ DNA Fragmentation Assay Kits |
| 20. Ready-to-use Apoptosis Inducers and Set |
| 21. Cell Proliferation Assay Kit |
| 22. LDH-Cytotoxicity Assay Kit |
| 23. Live-Dead Cell Staining Kit |
| 24. Growth Factors, Cytokines, and Chemokines |
| 25. Antibodies to Cytokines & Chemokines |
| 26. Anti-GFP Polyclonal Antibody |
| 27. Neuroscience Related Antibodies |
| 28. 5 Minute DNA Ligation Kit |
| 27. 20 Minute Gel Staining/Destaining Kit |
