You are here:Home
»
Kits and Assays
»
Kits for Apoptosis, BioAssay™
»
Caspase 8 Apoptosis Detection, Fluorometric BioAssay™ Kit (FLICE, MASH, Mch5)
Caspase 8 Apoptosis Detection, Fluorometric BioAssay™ Kit (FLICE, MASH, Mch5)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. USBio's Caspase-8 Apoptosis Detection Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light. | | | Catalog # | C2087-30 | | Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity. | | Kit Contents | Assays 25 100 200 400 | | Cell Lysis Buffer 25ml 100ml 100ml 100ml | | 2X Reaction Buffer 2ml 4x2ml 16ml 32ml | | IETD-AFC (1mM) 0.125ml 0.5ml 2x.05ml 2x1ml | | DT (1M) 0.1ml 0.4ml 0.4ml 0.4ml | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
