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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 8 Apoptosis Detection, Fluorometric BioAssay™ Kit (FLICE, MASH, Mch5)

Caspase 8 Apoptosis Detection, Fluorometric BioAssay™ Kit (FLICE, MASH, Mch5)

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Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. USBio's Caspase-8 Apoptosis Detection Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light.
Catalog #C2087-30
Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.
Kit ContentsAssays 25 100 200 400
Cell Lysis Buffer 25ml 100ml 100ml 100ml
2X Reaction Buffer 2ml 4x2ml 16ml 32ml
IETD-AFC (1mM) 0.125ml 0.5ml 2x.05ml 2x1ml
DT (1M) 0.1ml 0.4ml 0.4ml 0.4ml
Storage and Stability Store kit at -20°C before use. After opening, store Cell Lysis Buffer and 2X Reaction Buffer at 4°C. All reagents are stable for 6 months under proper storage conditions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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