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Caspase 8 Apoptosis Detection, Colorimetric BioAssay™ Kit (FLICE, MASH, Mch5)
Caspase 8 Apoptosis Detection, Colorimetric BioAssay™ Kit (FLICE, MASH, Mch5)
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| Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase 8 Apoptosis Detection, Colorimetric BioAssay™ Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity. | | | Catalog # | C2087-40 | | Kit Components | 25 assays 100 assays 200 assays | | C2087-40A: 2X Reaction Buffer 2ml 4x2ml 16ml | | C2087-40B: IETD-pNA (4mM) 125ul 500ul 2x500ul | | C2087-40C: DTT (1M) 100ul 400ul 400ul | | C2087-40D: Cell Lysis Buffer 25ml 100ml 100ml | | C2087-40E: Dilution Buffer 25ml 100ml 200ml | | Storage and Stability | Store kit at -20°C before use. After opening, store Cell Lysis Buffer (C2087-40D), 2X Reaction Buffer (C2087-40A) and Dilution Buffer (C2087-40E:) at 4°C. | | Note: Protect IETD-pNA (C2087-40B) from light | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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