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Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE, MASH, Mch5)
Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE,
MASH, Mch5)
Pricing
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| Colorimetric substrate for Caspase-8. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. See Caspase-8 Apoptosis Detection Kit C2087-40 which provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-pNA (p-nitroanilide). | | | Catalog # | C2087-41 | | Upon proteolytic cleavage of the substrate by caspases, free pNA is detected at a wavelength of 400nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the increase in Caspase-3 activity. | | FLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (lmax 400nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader. | | Peptide sequence | Ac-Ile-Glu-Thr-Asp-pNA | | Molecular Formula | C H N O | | Molecular Weight | 638.6 | | Purity | 95% (HPLC) | | Form | Supplied as a liquid in DMSO (4mM). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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