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You are here:Home » Molecular Biology » MB-Apoptosis, Caspase » Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE, MASH, Mch5)

Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE,
MASH, Mch5)

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Specifications

Colorimetric substrate for Caspase-8. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. See Caspase-8 Apoptosis Detection Kit C2087-40 which provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-pNA (p-nitroanilide).
Catalog #C2087-41
Upon proteolytic cleavage of the substrate by caspases, free pNA is detected at a wavelength of 400nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the increase in Caspase-3 activity.
FLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (lmax 400nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader.
Peptide sequence Ac-Ile-Glu-Thr-Asp-pNA
Molecular FormulaC H N O
Molecular Weight638.6
Purity95% (HPLC)
FormSupplied as a liquid in DMSO (4mM).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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