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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (CPP32, YAMA, Apopain) (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (CPP32, YAMA, Apopain) (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH
Caspase-3 is a 32kD protein, activated during apoptosis by cleavage into 17kD and 11kD fragments.
In immunohistochemical studies Caspase-3 expression has been shown to be widespread, but not present in all cell types. Differences in the level of Caspase-3 have been reported in cells of short-lived nature (e. g. germinal center B cells) and those that are long lived (e. g. mantle zone B cells).
Catalog #C2087-59
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Paraffin): 1:50-1:250. Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blot: 1:500
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1903-30U Sheep anti Rabbit IgG: HRP
Recommended Positive Control TissueLymph node/kidney
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.1% sodium azide, before the addition of glycerol to 40%.
PuritySerum.
ImmunogenRecombinant human caspase-3.
SpecificityRecognizes the both intact Caspase-3 and the 17kD fragment of Caspase-3 cell death protease.Does not recognizesthe 11kD fragment.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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