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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 9, Apoptosis Detection, Fluorometric BioAssay™ Kit (ICE-LAP6, Mch6, Apaf 3)

Caspase 9, Apoptosis Detection, Fluorometric BioAssay™ Kit (ICE-LAP6, Mch6, Apaf 3)

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Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light ( max 400nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence ( max 505nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
Catalog #C2088-05
Components 25 assays 100 assays
Cell Lysis Buffer 25ml 100ml
2X Reaction Buffer 2ml 4 x 2ml
LEHD-AFC Substrate (1mM) 125ul 500ul
DTT (1M) 100ul 400ul
StabilityStore kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions.
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4. Caspase-2, -3, -6, -8, -9 Colorimetric Protease Assay Kits and Reagents
5. Active Recombinant Human Caspases
6. Caspase Inhibitors and Inhibitor Set
7. Quick Detection of Apoptotic DNA Ladder Kit
8. In Situ DNA Fragmentation Detection Kits and Reagents
9. Apoptosis Inducers
10. Quick Cell Proliferation Assay Kit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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