Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 9 Apoptosis Detection, Colorimetric BioAssay™ Kit (ICE-LAP6, Mch6, Apaf 3)

Caspase 9 Apoptosis Detection, Colorimetric BioAssay™ Kit (ICE-LAP6, Mch6, Apaf 3)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase 9 Apoptosis Detection, Colorimetric BioAssay™ Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.
Catalog #C2088-10
Kit Components 25 assays 100 assays
C2088-10A: Cell Lysis Buffer 1 x 25ml 1 x 100ml
C2088-10B: 2X Reaction Buffer 1 x 2ml 4 x 2ml
C2088-10C: LEHD-pNA Substrate (4mM) 1 x 125ul 1 x 500ul
C2088-10D: DTT (1M) 1 x 100ul 1 x 400ul
C2088-10E: Dilution Buffer 1 x 25ml 1 x 100ml
Storage and StabilityStore kit at -20°C. Store Cell Lysis Buffer (C2088-10A), 2X Reaction Buffer (C2088-10B) and Dilution Buffer (C2088-10E) at 4°C after opening. Note: Protect LEHD-pNA (C2088-10C) from light. All reagents are stable for 6 months.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links