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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 10 (FLICE2, Mch4)

Anti -Caspase 10 (FLICE2, Mch4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Caspases are death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling pathways. All known isoforms of caspase-10 are abundantly expressed in fetal lung, kidney, and skeletal muscle but are very poorly expressed or absent in these tissues in the adult, implying a possible role for the caspase-10 family in fetal development.
Catalog #C2088-45
Caspase-10 belongs to DED (death effector domain) containing caspases and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2–4). Upon death ligand–receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1–4). This complex processes procaspase-10 into a large active subunit and a small subunit. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death (3–6).
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (KLH coupled) corresponding to residues around Asp219 of human caspase-10.
SpecificityDetects endogenous levels of full length caspase-10 and its various isoforms. Does not crossreact with other caspases. Recognizes human, mouse, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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