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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 12

Anti -Caspase 12

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IC
Caspase 12 is located in the endoplasmic reticulum (ER) subcellular compartment. It is responsible for apoptosis induced through ER stresses such as high calcium concentration, low oxygen and low glucose levels. One of the mechanisms for caspase 12 activation is related to calpain mediated caspase 12 cleavage at T132 and K158, both of which are located at the N-terminal part of the protein. Caspase 12 also has a putative caspase cleavage site located at the C-terminal part of the protein. In cortical neurons, caspase 12 is involved at least partially in the amyloid-beta neurotoxicity process.
Catalog #C2088-58
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunocytochemistry (ABC): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity and immunoaffinity chromatography.
ImmunogenSynthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 158 of mouse caspase-12.
SpecificityRecognizes mouse caspase 12 protein. Detects endogenous levels of full length caspase 12 protein (55kD) and its cleaved product (42kD). It does not crossreact with other caspase family members.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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