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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 14

Anti -Caspase 14

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-14 is a newly identified member of Caspase family. It has conserved active site pentapeptide, QACRG. However, no proteolytic or biological activities have been identified so far. It's high expression in embryonic tissues and limited expression in adult tissues suggests that it may have some role during ontogenesis.
Catalog #C2089-90
ApplicationsSuitable for detection of mouse caspase-14 by Western blotting. Whole cell lysate from mouse spleen tissue can be used as positive control and a 30kD band is detected. Western blot analysis for Caspase-14 at 2ug/ml dilution against 10ug of mouse spleen cell lysate.
Recommended DilutionWestern blot: 2-4ug/ml
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H47
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to amino acids 2-18 of mouse caspase-14.
SpecificityRecognizes mouse Caspase 14
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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