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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH

b-Catenin (~92kD) shares 70% amino acid identity with both plakoglobin (g-catenin) and the product of the Drosophila segment polarity gene armadillo. b-catenin, plakoglobin (g-catenin), and p120 cas are homologous but distinct proteins which contain between 10-13 copies of a 42-44 amino acid motif rst identi ed in the armadillo protein and referred to as armadillo repeats. These armadillo repeats mediate b-catenin’s interaction with the tumor suppressor protein APC (Adenomatous Polyposis Coli).
Catalog #	C2096-59
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Frozen and formalin-fixed, paraffin embedded tissue sections. Epitope retrieval required.
	Optimal dilutions to be determined by the researcher.
Recommended Positive Control Tissue	Non-neural epithelial tissue
Expected Staining Pattern	Membrane and cytoplasmic
	Epitope Retrieval: Required
	Enzyme Digestion: Not required
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3G67
Host	Mouse
Source	Chicken
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Puri ed from mouse ascites
Immunogen	Recombinant protein corresponding to the C-terminus of chicken b-catenin.
Specificity	Recognizes chicken b-catenin (~92kD) and does not crossreact with the related plakoglobin (~83kD) protein. Species Crossreactivity: human, mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.