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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta

Anti -Catenin, beta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #C2096-60
Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates early embryonic development (2) and tumorigenesis (3). GSK-3 beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5).
Applications Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Paraffin sections). Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (KLH coupled) corresponding to residues around Ser37 of human beta-catenin.
SpecificityThis antibody will only recognize beta-catenin and will not detect endogenous levels of alpha-catenin or gamma-catenin. Species Crossreactivity: Mouse and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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