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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Catalog #	C2096-60
Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates	early embryonic development (2) and tumorigenesis (3). GSK-3 beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5).
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Paraffin sections). Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunoprecipitation: 1:200
	Immunohistochemistry (Paraffin): 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide (KLH coupled) corresponding to residues around Ser37 of human beta-catenin.
Specificity	This antibody will only recognize beta-catenin and will not detect endogenous levels of alpha-catenin or gamma-catenin. Species Crossreactivity: Mouse and rat.
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.