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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta

Anti -Catenin, beta

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. Beta-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
Catalog #C2096-60A
Comments This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
Epitope C-terminal.
ApplicationsWestern Blot: (Ab 2ug/ml for 2 hours at RT)
Immunopricipitation
Immunohistology (Formalin/paraffin): (Ab 1:250 for 10 min at RT) [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]
Positive Control MCF-7 cells. Breast carcinoma
Cellular Localization Cytoplasmic and cell membrane
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide from the C-terminus of the beta-catenin protein.
SpecificityRecognizes beta Catenin. Species Crossreactivity: Human and Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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