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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. Beta-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
Catalog #	C2096-60A
Comments	This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
Epitope	C-terminal.
Applications	Western Blot: (Ab 2ug/ml for 2 hours at RT)
	Immunopricipitation
	Immunohistology (Formalin/paraffin): (Ab 1:250 for 10 min at RT) [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]
Positive Control	MCF-7 cells. Breast carcinoma
Cellular Localization	Cytoplasmic and cell membrane
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide from the C-terminus of the beta-catenin protein.
Specificity	Recognizes beta Catenin. Species Crossreactivity: Human and Mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.