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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta, phosphorylated (Tyr86)

Anti -Catenin, beta, phosphorylated (Tyr86)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
Catalog #C2096-63
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml for HRPO/ECL. Recommended blocking buffer: Casein/Tween 20 based blocking and incubation buffer.
ELISA: 0.05ug/ml.
Optimal dilutions to be determined by the researcher.
Positive Control IncludedC2096-63A: Catenin, beta, phosphorylated (Tyr86), Positive Control (SW480 Cell Lysate, Pervanadate Treated).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D229
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% sodium azide, PEG, Sucrose.
PurityPurified by subsequent thiophilic adsorption and size exclusion chromatography.
ImmunogenSynthetic peptide corresponding to a portion of b-catenin phosphorylated at Tyr86. Conjugated to hemocyanin. Epitope: phosphotyrrosine 86, DGQpYAMT.
SpecificityRecognizes human b-Catenin phosphorylated at Tyr86. Detects a band of ~90kD by Western Blot. Species Crossreactivity: mouse, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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