| The a-, b- and g-catenins are cytoplasmic proteins mediating the interaction of Ca-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. |
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| Additionally, b-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGF receptor and src family kinases while tyrosine 142 is a substrate of for tyrosine kinases. |
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| Catalog # | C2096-64 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1ug/ml (HRP/ECL). Recommended blocking buffer: CPPT (0.5% casein, 1% PEG 4000, 1% Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 2X PBS) |
| Optimal dilutions to be determined by the researcher. |
| Positive cell lysate control included | C2096-64A: Recommended for Western Blot at 20ul/lane. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6D230 |
| Host | Mouse |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, 0.1% sodium azide, PEG, Sucrose. |
| Purity | Purified by thiophilic adsorption and size exclusion chromatography. |
| Immunogen | Synthetic peptide corresponding to a portion of beta-catenin phosphorylated at Tyr654. Epitope: phosphotyrosine 654 (VATpYAAA) |
| Specificity | Recognizes beta-catenin phosphorylated at Tyr654 and -catenin phosphorylated at Tyr654. Detects bands of ~90kD (beta-catenin) and ~70kD (gamma-catenin). Due to sequence homologies of the phospho-epitope surrounding Tyr654, there may be crossreactions with other proteins (e.g., Annexin A11 at 56kD, desmoplakin at 81kD, TNF-alpha-induced protein 2 at 73kD, ribosomal protein S6 kinase alpha 6 at 90kD). Species Crossreactivity: human, mouse and canine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
