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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin B

Anti -Cathepsin B

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH FC
Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31kD or the two-chain form (25 and 5kD subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
Catalog #C2097-03
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-4ug/ml
Flow Cytometry: 1:50
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS NumberN/A
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 0.5% BSA, 30% glycerol
PurityPurified by affinity chromatography
ImmunogenSynthetic peptide surrounding aa322 of human cathepsins B
SpecificityRecognizes both the pro- (37-43kD) and mature (25kD) forms of human cathepsin B. Species Crossreactivity: mouse, rat (weak).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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