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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Cathepsins are localized primarily in lysosomes or endosomes, but translocated to the cytosol during apoptosis. The translocation allows these proteases to interact with caspases or other apoptosis signaling mechanisms. Studies show that cathepsins cleave certain caspases, and caspase inhibitors can inhibit cathepsin activity in vitro. Cathepsin B is a normal lysosomal proteinase that is expressed in all cells. It is a cysteine endoproteinase structurally and functionally related to the papain family of proteases. Cathepsin B is synthesized as an inactive 43kD pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31kD or the two-chain form (25 and 5kD subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown to be capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteases, the cystatins and stefins, may play a major role in regulating their activity. In tumor cells and in cells exposed to mitogens, cathepsin B displays altered cellular trafficking resulting in the secretion of the 43kD precursor. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
Catalog #	C2097-03
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 0.5-4ug/ml
	Immunohistochemistry: 10-20ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% thimerosal, 0.5% BSA, 30% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide surrounding aa322 of human cathepsins B.
Specificity	Recognizes both the pro- (37-43kD) and mature (25kD) forms of human cathepsin B. Species Crossreactivity: mouse, rat (weak).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.