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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin B

Anti -Cathepsin B


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Cathepsin B is a lysosomal cysteine proteinase that is important for intracellular protein catabolism. Cathepsin B has been implicated in several disease states including muscular dystrophy, rheumatoid arthritis and tumor metastasis.
Catalog #C2097-05
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommnended DilutionsWestern Blot: 2-4ug/ml. Detected both procathepsin B and cathespin B in 20ug of rat kidney microsomal preparation (M3893-27). Rat kidney microsomal preparation was resolved by electrophoresis, transferred to PVDF and probed with C2097-05. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: 10ug/ml positively stained rat kidney and liver sections that had been fixed with ethanol:acetic acid [95:5] for 5 minutes at RT.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to rat pro-Cathepsin B
SpecificityRecognizes rat pro-Cathepsin B (~40kD) and mature Cathepsin B (25, 26 and 30kD). Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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