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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin B

Anti -Cathepsin B

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Cathepsin B plays a role in protein degradation and has also been shown to activate urokinase type plasminogen activator (uPA) and certain matrix metalloproteinases (MMPs).
Catalog #C2097-11
ApplicationsSuitable for use in Immunohistochemistry. Not suitable for use in Western Blot or frozen sections. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:40-1:80 No pretreatment.
Optimal dilution determined by the researcher.
HybridomaSpleen cells form immunized mice were fused with cells of the p3-NSI-Ag4-1 myeloma cell line.
Recommended Secondary ReagentsF(ab')2 rabbit anti mouse IgG:HRP
Recommended Positive Control TissueLiver
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A221
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, 0.09% sodium azide. Reconstitute with 1ml dH2O or 40-50% glycerol..
PuritySupernatant
ImmunogenRecombinant human cathepsin B.
SpecificitySpecific for Cathspsin B, a lysosomal protease expressed in a wide range of tissues, including liver, skeletal muscle and heart.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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