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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

Cathepsin B plays a role in protein degradation and has also been shown to activate urokinase type plasminogen activator (uPA) and certain matrix metalloproteinases (MMPs).
Catalog #	C2097-11
Applications	Suitable for use in Immunohistochemistry. Not suitable for use in Western Blot or frozen sections. Other applications not tested.
Recommended Dilution	Immunohistochemistry (paraffin): 1:40-1:80 No pretreatment.
	Optimal dilution determined by the researcher.
Hybridoma	Spleen cells form immunized mice were fused with cells of the p3-NSI-Ag4-1 myeloma cell line.
Recommended Secondary Reagents	F(ab')2 rabbit anti mouse IgG:HRP
Recommended Positive Control Tissue	Liver
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	6A221
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder in PBS, 0.09% sodium azide. Reconstitute with 1ml dH2O or 40-50% glycerol..
Purity	Supernatant
Immunogen	Recombinant human cathepsin B.
Specificity	Specific for Cathspsin B, a lysosomal protease expressed in a wide range of tissues, including liver, skeletal muscle and heart.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.