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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)

Anti -Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D. Cathepsin D has been reported to be involved in malignant tumor progression and with prognosis of various human cancers.
Catalog #C2097-24B
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:250-1:1000
Immunohistochemistry: 1:50-1:100.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control TissueMelanoma, invasive ductal carcinoma
HybridomaDerived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cathepsin D protein.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No6j3
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human Cathepsin D (21-412 aa) purified from E. coli
SpecificityRecognizes human Cathepsin D at 45kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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