| Catalog # | C2097-33 |
| This kit is optimized to detect Cathepsin D activity. Enhanced Value | It provides enough reagents to perform 100 assays in a 96-well format. |
| High Speed | The entire process can be completed in one hour |
| Cathepsin D is the lysosomal aspartic proteinase implicated in intracellular protein degradation. It is involved in several pathological processes, such as inflammatory states, atherosclerosis, thrombosis, apoptosis, neoplastic proliferation or Alzheimer disease. 1-5 |
| The Cathepsin D Assay Kit provides a convenient assay for screening of enzyme inhibitors and for continuous assay of Cathepsin D activity using a Mca/Dnp FRET peptide. The sequence of this FRET peptide is derived from the cleavage site of Cathepsin D.6, 7 In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm. |
| Kit Components | Component A Microtiter Plate 1x100 wells Mca/Dnp, Cathepsin D substrate, Ex/Em=330 nm/390nm upon cleavage 1.6mM, 1x50ul |
| Component B Mca fluorescence reference standard, Ex/Em=330nm/390nm 2mM, 1x10ul |
| Component C Cathepsin D, bovine spleen 0.1mg/ml, 1x20ul |
| Component D Assay Buffer 1x20ml |
| Component E Pepstatin A 4mM, 1x100ul |
| Component F DTT 1 M, 1x20ul |
| Materials Required (but not provided) | • 96-well microplate: Black microplates provide better signal to noise ratio. |
| • Fluorescence microplate reader: Capable of detecting emission at 390 nm with excitation at 330 nm. |
| Storage and Handling | • Store all kit components at -20°C, except for Component C |
| • Store Component C at -80°C |
| • Protect Components A and B from light and moisture. |
| • Component D can be stored at room temperature for convenience. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
