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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Cathepsin D, Fluorimetric 390, BioAssay™ Kit (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)

Cathepsin D, Fluorimetric 390, BioAssay™ Kit (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl
peptidase, MGC2311)

Pricing

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Specifications

Catalog #C2097-33
This kit is optimized to detect Cathepsin D activity. Enhanced Value It provides enough reagents to perform 100 assays in a 96-well format.
High Speed The entire process can be completed in one hour
Cathepsin D is the lysosomal aspartic proteinase implicated in intracellular protein degradation. It is involved in several pathological processes, such as inflammatory states, atherosclerosis, thrombosis, apoptosis, neoplastic proliferation or Alzheimer disease. This kit provides a convenient assay for screening of enzyme inhibitors and for continuous assay of Cathepsin D activity using a Mca/Dnp FRET peptide. The sequence of this FRET peptide is derived from the cleavage site of Cathepsin D. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330nm/390nm.
Kit ComponentsC2097-33A: Cathepsin D substrate (1.6mM), Ex/Em=330nm / 390nm upon cleavage, 1x50ul
C2097-33B: Standard (2mM,), Ex/Em=330nm/390nm, 1x10ul
*C2097-33C: Cathepsin D, bovine spleen (0.1mg/ml), 1x20 ul
C2097-33D: Assay Buffer, 20ml
C2097-33E: Pepstatin A (4mM), 1x100ul
C2097-33F: DTT (1M), 1x100ul
Storage and StabilityStore *C2097-33C at -70°C. Store other components at 20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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