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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52kD pro-enzyme. Activation involves the proteolytic removal of the 43aa profragment and an internal cleavage to generate the two-chain form made up of 34 and 14kD subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52kD precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
Catalog #	C2097-36
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-4ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa140 from mouse cathepsins D.
Specificity	Recognizes mouse Cathepsin D at ~34kD and ~52kD. Species Crossreactivity: human and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.