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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin H

Anti -Cathepsin H

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Cathepsin H is a lysosomal glycoprotein and a member of the cysteine proteinase. This cysteine protease class comprises cathepsin B, H, K, L, and O. These proteinases are viewed as simple mediators of lysosomal protein degradation. Cathepsin H is synthesized as an inactive proenzyme (41kDa) and then proteolytically processed to an active single chain, mature form (28kDa) within the endosomes/lysosomes. This proteinase is a peptidehydrolase that firstly isolated from rat liver, and then identified in liver, kidney, spleen, and brain of different mammals. Furthermore, cathepsin H is increased in disease states including breast carcinoma, melanoma, gliomas, and colorectal and prostate carcinoma.
Catalog #C2097-61D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No7G23
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% thimerosal
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human cathepsin H (23-335 aa) (E. coli)
SpecificityRecognizes human Cathepsin H
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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