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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin L

Anti -Cathepsin L

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Cathepsins are localized primarily in lysosomes or endosomes, but translocated to the cytosol during apoptosis. The translocation allows these proteases to interact with caspases or other apoptosis signaling mechanisms. Studies show that cathepsins cleave certain caspases, and caspase inhibitors can inhibit cathepsin activity in vitro. Cathepsin L is a normal lysosomal protease that is expressed in all cells. It is a cysteine endoprotease that is structurally and functionally related to the papain family proteases. The Latent 39kD pro-Cathepsin L must be processed by cleavage of the 96 amino acid pro-peptide, to generate the active two-chain form made up of 21 and 5kD subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. Activity of Cathepsin L can be regulated by endogenous inhibitors called cystatins. The synthesis and secretion of the 39kD pro-Cathepsin L has been demonstrated for many human tumors, including cancers of the kidney, lung, colon, breast and ovary. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins suggests that it may play a role in invasion and metastasis and could be a useful prognostic indicator for cancer.
Catalog #C2097-72
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control J9000, Jurkat Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide surrounding amino acid 223 of human Cathepsin L.
SpecificityRecognizes both human mature and pro-Cathepsin L. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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