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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Cathepsin L [EC 3.4.22.15] is a lysosomal endopeptidase belonging to the papain family of the closely related cysteine proteases superfamily. The enzyme is ubiquitously distributed in the lysosomes of all cells and seems to be involved in a variety of disorders in which the pathological destruction of the extracellular matrix is one of the main characteristics.
Catalog #	C2097-74L
Applications
	Suitable for use in ELISA, Immunohistochemistry and Western blot. Other applications not tested.
Recommended Dilution	ELISA: Capture.
	Immunohistochemistry: paraffin sections after microwave treatment.
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D234
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS containing 0.01% thimerosal.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified human procathepsin L (from culture supernatant of non-small cell lung cancer cell line EPLC 32 M1).
Specificity	Recognizes the epitope aa265-271(GYGFEST) of human procathepsin L. Recognizes mature human single or two chain cathepsin L (heavy chain) and human procathepsin L. Cross-reacts with rat cathepsin L and weakly with mouse procathepsin L. Does not cross-react with mature cathepsins B, H, D, K, S and procathepsins B, D, F, S, W.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.