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You are here:Home » Antibodies » Antibodies-Cathepsin » Anti -Cathepsin S (Cathepsin-S, CATS, CTSS, FLJ50259, MGC3886)

Anti -Cathepsin S (Cathepsin-S, CATS, CTSS, FLJ50259, MGC3886)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH
Catalog #C2097-77D
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Formalin-fixed paraffin): 1:500
Optimal dilutions to be determined by the researcher.
Immunohistochemistry ProtocolTissue Sample: Mouse and rat visceral fat tissue
Fixative: 10% Formalin
Embedding: Paraffin
Negative control: Pre-immuno serum
Pretreatment: Intact
Blocking: 2% Normal Goat Serum
Primary Antibody: C2097-77D
Optimal Dilution: 1:500 (1 hour at RT)
Secondary Antibody: IgG (Biotin) GtxRb (1:400, 30 min)
Amplification: ABC (Vector) (1:400, 30 min)
Detection system: HRP
Substrate: DAB, 3 minutes
Counterstained: Hematoxylin, 30 seconds
Amino Acid SequenceAsn-Ser-Trp-Gly-His-Asn-Phe-Gly-Glu-Glu-Gly-Tyr-Ile-Arg-Met-Ala-Arg-Asn-Lys-Gly-Asn-His-Cys-Gly-Ile-Ala-Ser-Phe-Pro-Ser-Tyr-Pro-Glu-Ile
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa298-331 of human Cathepsin S.
SpecificityRecognizes human Cathepsin S. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.4.22.27


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