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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)

Anti -Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin
1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Catalog #C2097-91A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 2-8ug/ml Detects caveolin-1 and isoforms in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2097-71A (2ug/ml). Proteins were visualized using aIgG (HRP) GtxMo and a chemiluminescence detection system.
Immunoprecipitation: Immunoprecipitates caveolin-1 from rat adipocyte plasma membrane (1).
Immunocytochemistry: Detects caveolin-1 in 3T3-L1 adipocyte cells (1).
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlA0001-50: Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
*Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No4H312
HostMouse
SourceRat
Concentration~0.25mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenIntracellular membrane protein containing vesicles (containing GLUT4) from rat adipocytes.
SpecificityRecognizes c rat aveolin-1, Mr ~24kD (caveolin-1a) and 21kD (caveolin-1b) isoforms. A non-specific protein was also detected, Mr ~105kD. Species Crossreactivity: Human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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