Anti -Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin
1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IC |
|
| Catalog # | C2097-91A | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 2-8ug/ml Detects caveolin-1 and isoforms in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2097-71A (2ug/ml). Proteins were visualized using aIgG (HRP) GtxMo and a chemiluminescence detection system. | | Immunoprecipitation: Immunoprecipitates caveolin-1 from rat adipocyte plasma membrane (1). | | Immunocytochemistry: Detects caveolin-1 in 3T3-L1 adipocyte cells (1). | | Optimal dilutions to be determined by the researcher. | | Included Positive Antigen Control | A0001-50: Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. | | *Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 4H312 | | Host | Mouse | | Source | Rat | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Intracellular membrane protein containing vesicles (containing GLUT4) from rat adipocytes. | | Specificity | Recognizes c rat aveolin-1, Mr ~24kD (caveolin-1a) and 21kD (caveolin-1b) isoforms. A non-specific protein was also detected, Mr ~105kD. Species Crossreactivity: Human | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32) |
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