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Anti -CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome)
Anti -CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP IH IF |
|
| p300 is a transcriptional adapter protein, which is characterized by three cysteine- and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300 and p300-associated proteins, are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265kD nuclear protein designated CBP (for CREB- binding protein). CBP and p300 are homologous to each other. | | | Catalog # | C2098-03H | | Applications | Suitable for use in Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunoprecipitation: 2ug/mg protein lysate | | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | Tonsil, Ls174T cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b,k | | Clone No | SPM307 | | Host | Mouse | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | E1A affinity purified p300 protein. | | Specificity | Recognizes p300. Species Crossreactivity: human, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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