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You are here:Home » Antibodies » Antibodies-Disease Markers » Anti -CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome) (BSA & Azide Free)

Anti -CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS,
Rubinstein-Taybi Syndrome) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
p300 is a transcriptional adapter protein which is characterized by three cysteine-and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATAbinding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265kD nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other.
Catalog #C2098-03X
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher
Positive Control Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3F310
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2098-03.
PurityPurified by Protein A affinity chromatography.
ImmunogenE1A affinity purified p300 protein. Cellular Localization; Nuclear. MW of Antigen; 300kD. Epitope; aa2071-2091.
SpecificityRecognizes human p300 and CBP(1). p300 and CBP differ by three noncontiguous residues within the 21aa epitope region. A difference that does not detectably affect the reactivity of C2098-03. Species Crossreactivity: monkey, mink, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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