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Anti -CSK Binding Protein (CBP, Csk Binding Protein, PAG, Protein Associated with Glycosphingolipid Enriched Microdomains)
Anti -CSK Binding Protein (CBP, Csk Binding Protein, PAG, Protein Associated with
Glycosphingolipid Enriched Microdomains)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B FC |
|
| Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46kD transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80kD molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. | | | Catalog # | C2098-09A | | Positive Control | RAMOS and Jurkat cells | | Applications | Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 8J1093 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | 15mM Sodium Azide in PBS. pH 7.4 | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human Cbp/PAG. | | Specificity | This antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain. Species Crossreactivity: Crossreacts with Human. Does not react with mouse, rat, or bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | CBP, Csk Binding Protein, PAG, Protein Associated with Glycosphingolipid Enriched Microdomains |
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