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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -CCL26-Like (CCL26L, Eotaxin-3 Like)

Anti -CCL26-Like (CCL26L, Eotaxin-3 Like)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Chemokine (CC motif) ligand 26, also known as eotaxin 3, is a human CC chemokine ligand that binds and signals via the chemokine receptor CCR3. Rat CCL26-like/Eotaxin 3-like is an 8.5kD orthologue of the human CCL26/eotaxin 3. It is a 94 amino acid (aa) residue protein with a 23 aa signal sequence and a 71 aa mature segment. It is presumed that rat CCL26-like/Eotaxin 3-like will bind CCR3 to induce eosinophil chemotaxis and transendothelial migration. Mature rat CCL26 shares 48%, 63%, and 48% aa sequence identity with the human, mouse and canine CCL26/Eotaxin 3, respectively.
Applications:
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 1 ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 0.5ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Catalog #C2099-60
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceRat
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
PurityPurified by rat CCL26-like affinity chromatography.
ImmunogenE. coli-derived rrCCL26-like
SpecificitySelected for its ability to recognize rat CCL26L in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% crossreactivity with rmCCL26-like.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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