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Anti -CCL26-Like (CCL26L, Eotaxin-3 Like)
Anti -CCL26-Like (CCL26L, Eotaxin-3 Like)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Chemokine (CC motif) ligand 26, also known as eotaxin 3, is a human CC chemokine ligand that binds and signals via the chemokine receptor CCR3. Rat CCL26-like/Eotaxin 3-like is an 8.5kD orthologue of the human CCL26/eotaxin 3. It is a 94 amino acid (aa) residue protein with a 23 aa signal sequence and a 71 aa mature segment. It is presumed that rat CCL26-like/Eotaxin 3-like will bind CCR3 to induce eosinophil chemotaxis and transendothelial migration. Mature rat CCL26 shares 48%, 63%, and 48% aa sequence identity with the human, mouse and canine CCL26/Eotaxin 3, respectively. | | | | | | Applications: | | | Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 1 ng/lane under non-reducing and reducing conditions, respectively. | | | Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 0.5ng/well. | | | Optimal dilutions should be determined by each laboratory for each application. | | | Catalog # | C2099-60 | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Rat | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml. | | Purity | Purified by rat CCL26-like affinity chromatography. | | Immunogen | E. coli-derived rrCCL26-like | | Specificity | Selected for its ability to recognize rat CCL26L in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% crossreactivity with rmCCL26-like. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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