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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -CNOT4 (CCR4-NOT Transcription Complex Subunit 4, CCR4-associated Factor 4, E3 Ubiquitin-protein Ligase CNOT4, Potential Transcriptional Repressor NOT4Hp, NOT4)

Anti -CNOT4 (CCR4-NOT Transcription Complex Subunit 4, CCR4-associated Factor 4, E3
Ubiquitin-protein Ligase CNOT4, Potential Transcriptional Repressor NOT4Hp, NOT4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
CNOT4 is a 575aa protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.
Catalog #C2099-68W1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.05-0.1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman skeletal muscle lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa50-100 of human CNOT4.
SpecificityRecognizes human CNOT4. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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