| CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP 3 alpha). This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells. |
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| Catalog # | C2099-70B |
| Applications | Suitable for use in ELISA, Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:37,500 |
| Flow Cytometry: 2-6ul/10e6 cells in 100ul |
| Immunohistochemistry (Paraffin): 1:125 |
| Immunohistochemistry: 1:10-1:2000 |
| Western Blot: 1:250 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | |
| Human spleen or peripheral blood and mouse spleen |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa18-46, YFVSVNTSYYSVDSEMLLCSLQEVRQFSR of human CCR6. |
| Cellular Location | Integral membrane protein |
| Specificity | Recognizes human CCR6. Species Crossreactivity: mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
