| CCR7 is a member of the G protein-coupled receptor family (subfamily: chemokine). This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. It is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. |
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| Catalog # | C2099-71 |
| Applications | Suitable for use in Western Blot, ELISA, Immunohistochemistry (Formalin-fixed paraffin-embedded sections) and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Westen Blot: 1:1000 |
| ELISA: 1:100,000 |
| Immunohistochemistry (Paraffin): 1:250 |
| Immunocytochemistry: 1:500 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | IHC (P): Mouse spleen, human spleen |
| Western Blot: Cells transfected with CCR7 receptor. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide (DPGKPRKNVLVVALLVIFQVC) corresponding to aa2-22 of mouse CCR7. Peptide Sequence Homology |
| Specificity | Recognizes mouse CCR7. Species Crossreactivity: Human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
