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You are here:Home » Antibodies » Abs to G Proteins » Anti -CCR7 (Chemokine Receptor 7, CC-CCR-7)

Anti -CCR7 (Chemokine Receptor 7, CC-CCR-7)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC N
CCR7 is a 7TM G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.
Catalog #C2099-71M
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Neutralization. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-2.5x10e5 cells in a total volume 200ul.
Immunohistochemistry: 25ug/ml stains paraformaldehyde-fixed mouse splenocytes.
Neutralization: The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of C2099-71M was determined to be approximately 2-10ug/ml in the presence of 25ng/ml of rmMIP-3beta using the BaF/3 mCCR7 cell line in a chemoattraction assay.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8H212
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
Endotoxin<0.1EU/ug (LAL)
ImmunogenRecombinant corresponding to mouse CCR7 expressed in RBC-2H3 cells (NP_031745).
SpecificityRecognizes mouse CCR7.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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