| Receptors for the C-C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. The CCR8 receptor in particular mediates CC chemokine I 309 induced monocyte chemoattraction, HIV 1 envelope fusion and virus infection. |
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| Catalog # | C2099-72 |
| Applications | Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and Flow Cytometry/FACS. Other applications not tested. |
| Recommended Dilution | ELISA: 1:100,000 |
| FACS: 1.3ug/10e6cells/50-100ul |
| Immunocytochemistry: 1:500 |
| Immunohistochemistry (Paraffin): 1:400 |
| Western Blot: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | |
| Mouse spleen cells. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Mouse |
| Concentration | ~2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa7-33, PNVTMTDYYPDFFTAPCDAEFLLRGSM, of mouse CCR8. Cellular Localization: Cell membrane. |
| Specificity | Recognizes mouse CCR8 (50kD). Species sequence homology: < 50% identical to other mouse chemokine receptors in this region. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
