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You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841) (PE)

Anti -CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor,
CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841) (PE)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.
Catalog #C2099-92R
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat. Use 10ul per 10e6 cells
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of C2099-92E was added to up to 1x10e6 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells twice in staining buffer (BSA-supplemented saline buffer). Note: that whole blood requires a RBC lysis step at this point.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2a antibody.
Absorption 488nm
Emission 565-605nm
Storage and StabilityStore product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No11n21
HostRat
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein G affinity chromatography.
ImmunogenY3 rat myeloid cell line transfected with human CCR3
SpecificityRecognizes human CCR3. No cross­reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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