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C2099-93B Goat Anti-CCR4, NT (CC Chemokine Receptor Type 4, CCCKR4, CCR4, ChemR13, CKR4, CMKBR4, HGCN 14099, K5 5)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Mouse
Swiss Prot
P51679
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.

Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:25,000 Western Blot: 1:500 Immunohistochemistry: 1:250 Flow Cytometry: 2ul labels 10e6 cells in 100ul Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide DTTQDETVYNSYYFYESMPC corresponding to amino acid residues 8-26 of mouse CC chemokine receptor CCR4. Species sequence homology: other human chemokine receptors <50%.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse CCR4. No crossreactivity to human CCR4. Sequence is < 50 % identical to other human chemokine receptors in this region
References
1. Honjo A, Ogawa H, Azuma M, Tezuka T, Sone S, Biragyn A, Nishioka Y.Respir Investig. 2013 Dec;51(4):241-9.|2. Ferguson AR, Engelhard VH. J Immunol. 2010 Apr 15;184(8):4079-86. |3. Raimondi G, Sumpter TL, Matta BM, Pillai M, Corbitt N, Vodovotz Y, Wang Z, Thomson AW J. Immunol. 2010 Jan 15;184(2):624-36.|4. Guo Z, Jang MH, Otani K, Bai Z, Umemoto E, Matsumoto M, Nishiyama M, Yamasaki M, Ueha S, Matsushima K, Hirata T, Miyasaka M. Int Immunol. 2008 Mar;20(3):307-15. |5. Kanagawa N, Niwa M, Hatanaka Y, Tani Y, Nakagawa S, Fujita T, Yamamoto A, Okada N. Int J Cancer. 2007 Nov 1;121(9):2013-22.|6. Khader SA, Bell GK, Pearl JE, Fountain JJ, Rangel-Moreno J, Cilley GE, Shen F, Eaton SM, Gaffen SL, Swain SL, Locksley RM, Haynes L, Randall TD, Cooper AM.Nat Immunol. 2007 Apr;8(4):369-77.|7. Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K. Eur J Immunol. 2006 Aug;36(8):2116-27.|8. Ochando JC, Homma C, Yang Y, Hidalgo A, Garin A, Tacke F, Angeli V, Li Y, Boros P, Ding Y, Jessberger R, Trinchieri G, Lira SA, Randolph GJ, Bromberg JS. Nat Immunol. 2006 Jun;7(6):652-62.|9. Morimoto Y, Bian Y, Gao P, Yashiro-Ohtani Y, Zhou XY, Ono S, Nakahara H, Kogo M, Hamaoka T, Fujiwara H. J Leukoc Biol. 2005 Sep;78(3):753-61. |10. Jakubzick C, Wen H, Matsukawa A, Keller M, Kunkel SL, Hogaboam CM. Am J Pathol. 2004 Oct;165(4):1211-21 Full Text IH
USBio References
No references available
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