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You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G-Protein-coupled Receptor 2, GRP2) (APC)

Anti -CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G-Protein-coupled Receptor 2, GRP2)
(APC)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets, and functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.
Catalog #C2100-16A
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Neat; 10ul labels 1x10e6 cells. Validated for use with mouse B16F1 cells and rat splenocytes.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of mouse or rat IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C2100-16A to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final analysis by flow cytometry. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2B antibody.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at 12 months after receipt at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H55
HostRat
SourceMouse
Concentration~0.01mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
PurityPurified by Protein G affinity chromatography.
ImmunogenC6 cells transfected with mouse CCR10
SpecificityRecognizes mouse CCR10. Species Crossreactivity: rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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