| Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR11 in particular is expressed in the heart, lung, and small intestine. It binds members of the monocyte chemoattractant family including MCP-1, MCP-2, and MCP-4. |
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| Catalog # | C2100-20A |
| Application(s) | Suitable for use in Western Blot, ELISA, Immunohistochemistry (Formalin-fixed paraffin-embedded sections) and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:75,000 |
| Immunocytochemistry: 1:500 |
| Immunohistochemistry (Paraffin): 1:250 |
| Western Blot: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | |
| Human heart, lung or small intestine. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide: EQNQSTDYYYEENEMNGTYDYSQYELIC, corresponding to amino acids 4-31 of Human CCR11. |
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| Specificity | Recognizes human CCR11. Species Crossreactivity: Mouse. Peptide sequence is < 50% identical to other human chemokine receptors in this region. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
