| Catalog # | C2252-03E |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: Use 10ul to label 1x10e6 cells in 100ul. |
| Immunohistochemistry: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Spleen cells from immunized Biozzi mice were fused with cells of the NS1 mouse myeloma cell line. |
| Recommended Negative Controls | I1904-78R: IgG1 Murine Negative Control |
| Recommended Secondary Reagents | I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo |
| I1904-66A: IgG, F(ab')2 (FITC) Pab Rb xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H648 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.09% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Thymus cells. |
| Specificity | 3H648 recognizes a polypeptide (CD1c) antigen of 43kD which is associated with the 12kD beta 2 microglobulin present on thymocytes, a subpopulation of B-lymphocytes early during ontogeny, Langerhans cells and dendritic cells. It may be useful for studies of calcium flux induced by CD1c. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
