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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor) (Biotin)

Anti -CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep
Erythrocyte Receptor) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggerring of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukemias of T cell origin.
Catalog #C2253-07R
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry
Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen, Paraffin (see ref 2)
Flow Cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H657
HostMouse
SourceRat
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 1% BSA. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenActivated rat T helper cells.
SpecificityRecognizes the rat CD2 cell surface antigen, a 50-54kD glycoprotein expressed by thymocytes and mature T cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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