Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified IH

Human CD3, a glycoprotein, consists of five polypeptide chains (gp20, gp26, p16, p20 & p28). The CD3 complex is involved in T cell activation and signal transduction. The CD3 antigen is T cell specific, and normally present in resting and activated T lymphocytes. It is also present in peripheral blood T cells and thymocytes. CD3 antibody is specific for T cells, and often used to distinguish precursor T cell lymphoblastic lymphoma from its B cell counterpart.
Catalog #	C2254-02
Applications	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded tissue sections.
	Optimal dilutions to be determined by the researcher.
Epitope Retrieval	Not Required. HIER exhibited weaker staining than Pepsin pretreatment.
Enzyme Digestion	Required
Expected Staining Pattern	Membrane
Positive Control	Tonsil or Thymus
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Purified
Immunogen	Synthetic peptide derived from human CD3.
Specificity	Recognizes both normal and neoplastic human T cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.