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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD3 (Early T Cell Marker)

Anti -CD3 (Early T Cell Marker)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified IH
Human CD3, a glycoprotein, consists of five polypeptide chains (gp20, gp26, p16, p20 & p28). The CD3 complex is involved in T cell activation and signal transduction. The CD3 antigen is T cell specific, and normally present in resting and activated T lymphocytes. It is also present in peripheral blood T cells and thymocytes. CD3 antibody is specific for T cells, and often used to distinguish precursor T cell lymphoblastic lymphoma from its B cell counterpart.
Catalog #C2254-02
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Frozen and formalin-fixed paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.
Epitope RetrievalNot Required. HIER exhibited weaker staining than Pepsin pretreatment.
Enzyme DigestionRequired
Expected Staining PatternMembrane
Positive ControlTonsil or Thymus
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
PurityPurified
ImmunogenSynthetic peptide derived from human CD3.
SpecificityRecognizes both normal and neoplastic human T cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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