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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IH FC

CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation.
Catalog #	C2254-56N
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and In vitro activation. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 10ul/10e6 cells
	Immunohistochemistry: Frozen sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5K205
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS, sodium azide and a stabilizing agent. Labeled with APC.
Purity	Purified
Immunogen	Human CD3
Specificity	Recognizes the 20kD epsilon chain of the human CD3/T cell antigen receptor complex.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.