Anti -CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth
Factor Receptor) (PE/Cy5.5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Purified |
IP IH FC |
|
| CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested (1,2). CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors (3-6). The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells (1,2). In vivo and in vitro treatment with the effectively depletes CD4+ cells (7,8). The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines (1,8-13). | | | Catalog # | C2255-31H | | Applications | Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, In vivo and in vitro depletion of CD4+ cells, Inhibition of T helper function and Fractionation of CD4+ cells. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 0.2ug labels 10e6 cells | | Immunohistochemistry: Frozen sections | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin, Cyanine 5.5 (PE/Cy5.5) conjugates will result in a substantial loss of activity. PE/Cy5.5 conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b,k | | Clone No | 5K206 | | Host | Rat | | Source | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, stabilizing agent. Labeled with R-Phycoerythrin, Cyanine 5.5 (PE/Cy5.5). | | Purity | Purified | | Immunogen | Cloned mouse CTL line V41. | | Specificity | Mouse CD4/L3T4, Mr 52kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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