| CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) is a single chain 100kD glycoprotein, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. |
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| Catalog # | C2261-41A |
| Antigen distribution | Granulocytes > 95% cALL > 95% Peripheral blood lymphocytes < 1% T cells (lg+) < 1% B cells (lg-) < 1% Monocytes < 1% Thymocytes < 1% NK cells (CD16+) < 1% |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: Neat. 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | FC |
| I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo |
| Recommended Secondary Antibodies | IHC |
| I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo |
| I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb xMo |
| Negative Control | I1904-78R: IgG1 Negative Control |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line. |
| Recommended Negative Controls | I1904-78R: Mouse IgG1 Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H919 |
| Host | Mouse |
| Source | Human |
| Concentration | Not determined. |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40% |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Partially purified cell membrane antigens from fresh leukemia cells. |
| Specificity | Recognizes the human CD10 cell surface antigen, a 100kD glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes. CD10 is also known as the Common Acute Lymphoblastic Leukaemia Antigen (CALLA). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
