| The human CD10 cell surface antigen is a 100kD glycoprotein also known as CALLA (common acute lymphoblastic leukaemia antigen). CD10 is expressed on a variety of cell types, including immature and germinal centre B cells, lymphoblastic leukaemia cells and also on the brush border of kidney epithelial cells. |
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| Catalog # | C2261-41B |
| Applications | Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry (Frozen, paraffin): 1:80. Requires antigen retrieval using heat treatment with sodium citrate buffer pH 6.0 prior to staining paraffin sections. |
| Western Blot: 1:50-1:100 |
| Optimal dilutions to be determined by researcher. |
| Immunohistochemistry Positive Controls | Tonsil, intestine, kidney |
| Hybridoma | p3-NS1-Ag4-1 myeloma cells with spleen cells from mice. |
| Recommended Secondary Antibodies | I1904-04D: IgG, H&L (AP) Pab Gt xMo |
| I1904-06F: IgG, H&L (FITC) Pab Gt xMo |
| I1904-06C: IgG, H&L (HRP) Pab Gt xMo |
| I1903-07E: IgG, (HRP) Pab Rb xMo |
| I1903-13A: IgG, (PE) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H918 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid, 0.09% sodium azide. |
| Purity | Supernatant |
| Immunogen | Recombinant human CD10. |
| Specificity | Recognizes human CD10. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
