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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11)

Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral
Endopeptidase 24.11, EC3.4.24.11)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
The human CD10 cell surface antigen is a 100kD glycoprotein also known as CALLA (common acute lymphoblastic leukaemia antigen). CD10 is expressed on a variety of cell types, including immature and germinal centre B cells, lymphoblastic leukaemia cells and also on the brush border of kidney epithelial cells.
Catalog #C2261-41B
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen, paraffin): 1:80. Requires antigen retrieval using heat treatment with sodium citrate buffer pH 6.0 prior to staining paraffin sections.
Western Blot: 1:50-1:100
Optimal dilutions to be determined by researcher.
Immunohistochemistry Positive ControlsTonsil, intestine, kidney
Hybridomap3-NS1-Ag4-1 myeloma cells with spleen cells from mice.
Recommended Secondary AntibodiesI1904-04D: IgG, H&L (AP) Pab Gt xMo
I1904-06F: IgG, H&L (FITC) Pab Gt xMo
I1904-06C: IgG, H&L (HRP) Pab Gt xMo
I1903-07E: IgG, (HRP) Pab Rb xMo
I1903-13A: IgG, (PE) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H918
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySupernatant
ImmunogenRecombinant human CD10.
SpecificityRecognizes human CD10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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