| CD10 is a 100kD glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes. CD10 is also known as the Common Acute Lymphoblastic Leukaemia Antigen (CALLA). |
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| Catalog # | C2261-41E |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line. |
| Recommended Negative Controls | Mouse IgG1 Negative Control (Azide Free) |
| Recommended Secondary Reagents | I1904-65W IgG, F(ab’)2 (HRP) Pab Rb xMo |
| I1904-66C IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo |
| Rabbit anti mouse IgG: PE conjugate |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H919 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS. No preservatives added. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Partially purified cell membrane antigens from fresh leukemia cells. |
| Specificity | Recognizes the human CD10 cell surface antigen. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
