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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) (FITC)

Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral
Endopeptidase 24.11, EC3.4.24.11) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) is a single chain 100kD glycoprotein, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Catalog #C2261-41G
Antigen distribution Granulocytes > 95% cALL > 95% Peripheral blood lymphocytes < 1% T cells (lg+) < 1% B cells (lg-) < 1% Monocytes < 1% Thymocytes < 1% NK cells (CD16+) < 1%
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control: FITC
ApplicationsFlow Cytometry: Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H919
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenPartially purified cell membrane antigens from fresh leukemia cells.
SpecificityRecognizes the human CD10 cell surface antigen, a 100kD glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes. CD10 is also known as the Common Acute Lymphoblastic Leukaemia Antigen (CALLA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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