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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) (PE)

Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral
Endopeptidase 24.11, EC3.4.24.11) (PE)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) is a single chain 100kD glycoprotein, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Catalog #C2261-41H
Antigen distribution Granulocytes > 95% cALL > 95% Peripheral blood lymphocytes < 1% T cells (lg+) < 1% B cells (lg-) < 1% Monocytes < 1% Thymocytes < 1% NK cells (CD16+) < 1%
ApplicationsSuitable for use in Flow Cytometry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: Neat. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul
Optimal working dilutions to be determined by researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line.
Recommended Negative ControlsMouse IgG1 Negative Control: RPE
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H919
HostMouse
SourceHuman
Concentration~100 Tests/1ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartially purified cell membrane antigens from fresh leukemia cells.
SpecificityRecognizes the human CD10 cell surface antigen, a 100kD glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes. CD10 is also known as the Common Acute Lymphoblastic Leukaemia Antigen (CALLA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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