Anti -CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral
Endopeptidase 24.11, EC3.4.24.11) (PE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC IF |
|
| CD10 (CALLA, Common Acute Lymphocytic Leukemia Antigen, Neprilysin, NEP, Neutral Endopeptidase 24.11, EC3.4.24.11) is a single chain 100kD glycoprotein, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. | | | Catalog # | C2261-41P | | Antigen distribution | Granulocytes > 95% cALL > 95% Peripheral blood lymphocytes < 1% T cells (lg+) < 1% B cells (lg-) < 1% Monocytes < 1% Thymocytes < 1% NK cells (CD16+) < 1% | | Applications | Suitable for use in direct Immunofluorescence for Flow Cytometric analysis. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 20ul/1x10e6 cells or 100ul peripheral blood cells. | | Optimal dilution determined by the researcher. | | Storage and Stability | May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5F12 | | Host | Mouse | | Source | Human | | Concentration | 40ul/test | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.1% sodium azide before the addition of glycerol to 40%. Conjugated to PE. | | Purity | Purified by Protein G affinity chromatography. | | Specificity | Recognizes neutral endopeptidase, a 100kD type II transmembrane glycoprotein, which is refered as the Common Acute Lymphoblastic Leukemia Antigen (CALLA). Reacts with normal human peripheral blood granulocytes (99%) and REH cell (94%), is negative in peripheral blood lymphocytes and monocytes. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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